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205 nm UV-Vis readings

205 nm UV-Vis readings


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Typically we determine the concentration of proteins using a 280 nm reading. However, it is reasonable to use 205 nm. I was curious about the effectiveness of this method.


This may be what you are looking for. The conversion formula is:

$$ ext{Protein concentration (mg/ml)} = frac{ ( ext{Absorbance at 205 nm})} {31} $$

The first, Scopes, does indicate that nucleic acid contaminants will confounding 205 nm absorbance readings. It seems as though this spetrophotometic calculation is feasible for relatively pure protein species.


Watch the video: UV SPECTROSCOPY (July 2022).


Comments:

  1. Nereus

    You are wrong. I'm sure. I propose to discuss it.

  2. Deavon

    Hmm ... Nothing at all.

  3. Brademagus

    Moscow was not built at once.



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